Fisk will miss the Lionesses' friendly against Ghana on Tuesday night and has returned to the AXA Melwood Training Centre for assessment.
An England statement read: "Grace Fisk has withdrawn from the England squad with a small calf injury."
The defender was an unused substitute in Sarina Wiegman's side's 8-0 win over China on Saturday night.
In a press conference on Monday, Wiegman said: "Unfortunately Grace Fisk picked up a small injury so she will not be available for tomorrow."
